Optimization of total RNA isolation from Cochlodinium polykrikoides

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

Estimation of Cochlodinium polykrikoides growth potential using a dialysis membrane.

We developed a test to measure the growth potential of C. polykrikoides using a dialysis membrane and artificial seawater. Nitrite nitrogen and inorganic phosphorus in the medium were almost completely removed when the medium was dialyzed against artificial seawater for five or more 6-hour cycles using a dialysis membrane (Spectrum's Spectra/Por 7 Membrane) with a molecular-weight cut-off of 50...

Survival and growth of Cochlodinium polykrikoides red tide after addition of yellow loess.

We examined the survival rate of Cochlodinium polykrikoides after yellow loess addition and conducted culture experiments to investigate the possibility that red tides maybe caused by C. polykrikoides individuals that are precipitated when loess is added. At least 15% of the C. polykrikoides cells that precipitated to the bottom layer either by the addition of loess or no addition survived for ...

Real time detecting of harmful dinoflagellate Cochlodinium polykrikoides using unmanned surface vehicle in dynamic environments.

Since the first occurrence in 1982, red tides have been observed annually in Korean coastal waters in the form of harmful dinoflagellate Cochlodinium polykrikoides blooms. The distinction in the proposed method for red tide monitoring is the focus on the narrow stripe red tide at an early stage to allow for advanced actions. The distance graph between Head of Narrow Red tide (HNR) and location ...

Biochemical responses of fish exposed to a harmful dinoflagellate Cochlodinium polykrikoides.

To elucidate the ichthyotoxic mechanisms of a harmful dinoflagellate Cochlodinium polykrikoides, biochemical responses of fish exposed to blooms were investigated. Particularly, based on our finding that oxidative damages of gill were associated with fish mortality (J. Plankton Res. 21 (1999) 2105-2115), dysfunction of ion-transporting enzymes and secretion of gill mucus of fish exposed to this...